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Fig. 2. Autoradiogram (left side) and corresponding fluorescence image (right side) following 10-d autoradiographic film exposure to cells from 36-h aerobic incubations of polycyclic aromatic hydrocarbon contaminated aquifer sediments with [9-14C]phenanthrene. Silver grain clusters surrounding bacterial cells indicate active cellular incorporation of [9-14C]phenanthrene (A and B), which can be differentiated from bacteria that do not metabolize [9-14C]phenanthrene (C and D). Pink cells indicate Bacteria [EUB338(I, II, III) and blue cells are DAPI stained. Bar in autoradiogram = 10 µm. MAR = microautoradiography.
