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Published online 16 May 2007
Published in Soil Sci Soc Am J 71:1045-1050 (2007)
DOI: 10.2136/sssaj2006.0295
© 2007 Soil Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Right arrow Phosphorus
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Right arrow Nuclear Magnetic Resonance, NMR

Identification of Phytate in Phosphorus-31 Nuclear Magnetic Resonance Spectra: The Need for Spiking

Ronald J. Smernik* and Warwick J. Dougherty

Soil and Land Systems, School of Earth and Environmental Sciences, Univ. of Adelaide, Waite Campus Urrbrae, SA, 5064, Australia


Figure 1
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  		Fig. 1. Phosphorus-31 nuclear magnetic resonance spectra of NaOH-EDTA soil extracts. The vertical scale has been increased by a factor of 10 in the inset spectra.

 

Figure 2
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  		Fig. 2. Phosphorus-31 nuclear magnetic resonance spectra of unspiked NaOH-EDTA soil extracts (bottom traces of each pair) and phytate-spiked (top traces) NaOH-EDTA soil extracts. *Phytate resonances. {dagger}Orthophosphate resonance.

 

Figure 3
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  		Fig. 3. Phosphorus-31 (31P) nuclear magnetic resonance (NMR) spectra used to determine the phytate concentration in the Camden soil. (a) 31P NMR spectrum of unspiked NaOH-EDTA extract. (b) Phosphorus 31 (31P) NMR spectrum of NaOH-EDTA extract spiked with phytate. (c) 31P NMR spectrum of phytate spike generated by subtracting a from b. (d) 31P NMR spectrum containing nulled phytate resonances generated by subtracting 0.2 x Spectrum c from Spectrum a.

 

Figure 4
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  		Fig. 4. Phosphorus-31 nuclear magnetic resonance spectra of an orthophosphate and phytate mixture at varying pH and ionic strength. {dagger}Orthophosphate resonance.

 




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