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Fig. 1. Diagram of primer locations in the ribosomal cassette (modified from Martin and Rygiewicz, 2005). Primers are positioned above (forward primers) or below (reverse) their sequence positions in the ribosomal cassette, consisting of SSU, ITS1, 5.8S, ITS2, and LSU rDNA. Primers ITS1, ITS2, ITS3, ITS4, and TW13 from White et al. (1990); Primers ITS8mun, ITS9mun, ITS10mun, NL5mun, NL6Amun, NL6Bmun, and NL8mun from Egger (1995); NL6C from Kernaghan et al. (2003); Primers ITS1-F, ITS4-B from Gardes and Bruns (1993); and the remaining primers (NSA3, NSI1, 58A1F, 58A2F, 58A2R, NLB4, NLC2) from Martin and Rygiewicz (2005).
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