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Published online 12 March 2007
Published in Soil Sci Soc Am J 71:572-578 (2007)
DOI: 10.2136/sssaj2006.0147
© 2007 Soil Science Society of America
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Assessing Microbial Community Diversity Using Amplicon Length Heterogeneity Polymerase Chain Reaction

DeEtta K. Millsa,*, James A. Entryb, Patrick M. Gillevetc and Kalai Matheed

a Dep. of Biological Sci. and Intl. Forensic Research Inst., Florida International Univ., Miami, FL 33199
b USDA-ARS, Northwest Irrigation and Soils Research Lab., Kimberly, ID 83341
c Dep. of Environmental Science and Policy, George Mason Univ., Manassas, VA 20110.
d Dep. of Biological Sci. and Intl. Forensic Research Inst., Florida International Univ., Miami, FL 33199


Figure 1
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Fig. 1. Length heterogeneity–polymerase chain reaction applied to the intergenic region between the amoC and the amoA genes of the ammonia monooxygenase operon. The top panel is the electropherogram from soil treated with composted manure, the middle panel is the electropherogram from fresh cow manure, and the bottom panel is unamended soil (no manure) where the genes are either below detection or absent. The numbers in the box are the lengths of the amplicons in base pairs.

 





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