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Identification of scyllo-Inositol Phosphates in Soil by Solution Phosphorus-31 Nuclear Magnetic Resonance Spectroscopy

^a USDA-ARS, Northwest Irrigation and Soils Research Laboration, 3793N, 3600E, Kimberly, ID 83341
^b CSIRO Plant Industry, P.O. Box 1600, Canberra, ACT 2601, Australia
^c Soil and Water Science Dep., Univ. of Florida, 106 Newell Hall, P.O. Box 110510, Gainesville, FL 32611

View larger version (9K): [in a new window]   Fig. 1. Structure of scyllo-inositol hexakisphosphate in solution: (a) the all equatorial structure at pH < 9.0, (b) the all axial structure at pH > 11.0 (Volkmann et al., 2002).

View larger version (20K): [in a new window]   Fig. 2. Spectra of scyllo-inositol phosphate standards synthesized by the late Dr. Dennis Cosgrove determined by solution 31P NMR spectroscopy. Compounds were analyzed at pH > 13. Phosphate groups responsible for the individual signals are indicated by the position of the phosphate on the inositol ring.

View larger version (40K): [in a new window]   Fig. 3. Solution 31P NMR spectra of NaOH–EDTA extracts of three temperate pasture soils from England and Wales. Extracts were analyzed untreated and after pretreatment by hypobromite oxidation to destroy all organic matter except the inositol phosphates. Brominated samples were analyzed after redissolving in either 1.0 M NaOH or 1.0 M NaOH plus 0.1 M EDTA. Chemical shifts are shown in ppm relative to an external H3PO4 standard. All spectra are plotted using a 5-Hz line broadening, except the inset spectra of the brominated extracts redissolved in NaOH plus EDTA, which are plotted with 1-Hz line broadening to preserve enhanced spectral resolution.