Release of Intracellular Solutes by Four Soil Bacteria Exposed to Dilution Stress
Larry J. Halversona,
Thomas M. Jonesb and
Mary K. Firestoneb
a Deps. of Agronomy and Microbiology, 2537 Agronomy Hall, Iowa State Univ., Ames, IA 50011-1010 USA
b Dep. of Environmental Science, Policy, and Management, 151 Hilgard Hall, Univ. of California, Berkeley, CA 94720-3110 USA
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Fig. 1 Release of cellular protein following dilution. The proportion of cellular constituents released to the supernatant was derived by dividing extracellular contents by total intra- and extracellular contents. This value was then used to calculate the percentage released by subtracting control values (no water potential change) from treatments in which there was a water potential increase. Values are the mean ± standard error of the mean of three replications
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Fig. 2 Release of amino acids following dilution. See legend for Fig. 1 for a description of the calculation of release values. Values are the mean ± standard error of the mean of three replications
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Fig. 3 Release of low molecular weight neutral sugars following dilution. See legend for Fig. 1 for a description of the calculation of release values. Values are the mean ± standard error of the mean of three replications
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Fig. 4 Effects of dilution on culturability of Pseudomonas chlororaphis and P. fluorescens. Values are the mean ± standard error of the mean of three replications. Closed symbols, trypticase soy agar; open symbols, glucose minimal agar
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Copyright © 2000 by the Soil Science Society of America.
