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Pseudomonas cepacia–Mediated Rock Phosphate Solubilization in Kaolinite and Montmorillonite Suspensions

B. Bar-Yosefa, R.D. Rogersb, J.H. Wolframb and E. Richmanb

a Agricultural Research Organization, Inst. of Soil, Water, and Environmental Sciences, Bet Dagan 50250, Israel
b Idaho National Engineering and Environmental Lab., Biotechnology Unit, Idaho Falls, ID 83415 USA



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Fig. 1 Protons and hydroxyls consumption (application minus addition in solution) by Ca–montmorillonite (CaMT) and K–montmorillonite (KMT) and Ca–kaolinite (CaKL) and K–kaolinite (KKL) (1:40 clay/electrolyte [0.01 M Cl-] suspensions) as a function of suspension pH

 


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Fig. 2 (a) Orthophosphate concentration (Cp) and (b) pH in solution of rock phosphate (no-clay) suspensions as a function of equilibration time. Inoculation took place at t = 24 h. An asterisk (*) represents a significant difference (t test comparison, P < 0.05) between biotic (E37) and abiotic (no E37) treatments at given time and initial pH treatment

 


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Fig. 3 (a) Orthophosphate concentration (Cp) and (b) pH in solution of Ca–kaolinite + rock-phosphate suspensions (1:40) as a function of time. Inoculation took place at t = 24 h. An asterisk (*) represents a significant difference (t test comparison, P < 0.05) between biotic (E37) and abiotic (no E37) treatments at given time and initial pH treatment

 


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Fig. 4 (a) Orthophosphate concentration (Cp) and (b) pH in solution of Ca–kaolinite (CaKL), Ca–montmorillonite (CaMT) and K–montmorillonite (KMT) suspensions (1:40) as a function of equilibration time. Rock phosphate was added to all suspensions at t = 0. Inoculation with E37 took place at t = 24 h (CaKL) or 6 h (CaMT, KMT)

 


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Fig. 5 Gluconic acid (GA) + 2-ketogluconic acid (KGA) concentration in solution of Ca–kaolinite (CaKL), Ca–montmorillonite (CaMT), and K–montmorillonite (KMT) suspensions (1:40) as a function of equilibration time. Phosphorus was added as rock phosphate at t = 0. Inoculation with E37 took place at t = 24 h (CaKL) or 6 h (CaMT, KMT)

 





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