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Nutrient Management & Soil & Plant Analysis

Production of Nitrogen-15-Labeled Pig Manure for Nitrogen Cycling Studies

Danish Institute of Agricultural Sciences, Dep. of Agroecology, P.O. Box 50, Reseach Centre Foulum, 8830 Tjele, Denmark

^* Corresponding author (peter.sorensen{at}agrsci.dk)

Nitrogen-15 labeled pig (Sus scrofa) manure was produced to study the homogeneity of labeled pig manure prepared for soil N cycling studies. Growing pigs were fed with a diet consisting of mainly ¹⁵N-labeled barley (Hordeum vulgare L.) and peas (Pisum sativum L.) during a period of 11 d. Labeled feces and urine were collected separately. The ¹⁵N enrichment of urine N was lower than that of fecal N except for the first 2 d of the ¹⁵N feeding period. After 11 d of ¹⁵N feeding, the ¹⁵N enrichment of fecal N was 7% lower and urine N 28% lower than the enrichment of feed N. The homogeneity of ¹⁵N-labeling of feces N, sampled at different times during the ¹⁵N feeding, was tested by an incubation test in quartz sand and soil. After 12 wk in soil at 20°C, 25 to 27% of the feces N was in inorganic form, and the N mineralization rate declined after this period. The test indicated that the ¹⁵N-labeled pig feces had a satisfactory labeling homogeneity even though the feed ingredients had variable ¹⁵N enrichments. We recommend the collection of urine and feces separately and the use of labeled feces in combination with unlabeled urine and vice versa in studies of the fate of pig manure N in agroecosystems. If feces and urine with a similar ¹⁵N enrichment can be obtained the two components can be mixed in the same manure.

Abbreviations: ANI, added-nitrogen interaction • PE, polyethylene

This article has been cited by other articles:

				P. Sorensen and I. K. Thomsen Separation of Pig Slurry and Plant Utilization and Loss of Nitrogen-15-labeled Slurry Nitrogen Soil Sci. Soc. Am. J., August 25, 2005; 69(5): 1644 - 1651. [Abstract] [Full Text] [PDF]