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a Stable Isotope Lab., Univ. of California-Davis, Davis, CA 95616
b Dep. of Land, Air, and Water Resources, Univ. of California-Davis, Davis, CA 95616
c Dep. of Agronomy and Range Science, Univ. of California-Davis, Davis, CA 95616
* Corresponding author (cvankessel{at}ucdavis.edu)
The use of 13C natural abundance (
13C) to follow C input to soil has gained widespread acceptance. However, inorganic C present in the soil as carbonates will interfere with the measurement of soil organic 13C unless removed or excluded from measurement. We report a simple and convenient HCl-fumigation method to remove inorganic C from soil. Soil samples are weighed in Ag-foil capsules, arranged on a microtiter plate, wetted with water to approximately field capacity, and placed in a desiccator containing a beaker with concentrated (12 M) HCl. The carbonates are released as CO2 by the acid treatment in 6 to 8 h. The soil samples are then dried at 60°C prior to isotope determination. The advantages of the HCl-fumigation method to remove inorganic C from the soil are that: (i) no water soluble C will be lost from the soil; (ii) a large number of samples can be processed simultaneously; (iii) the removal of inorganic C is rapid and complete; and (iv) the method could also be used to determine both organic and inorganic C content in the soil. A potential disadvantage, however, is that the HCl fumigation changed the 15N natural abundance of soil N.
Abbreviations: SOM, soil organic matter • FACE, Free Atmospheric CO2 Enrichment • HCl, HCl acid • 13C, natural abundance 13C
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