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Effect of Acetylene on Nitric Oxide Production in Soil under Denitrifying Conditions

Dep. of Chemistry and Biochemistry, Univ. of Windsor, Windsor, ON, Canada N9B 3P4

C. F. Drury

Agriculture and Agri-Food Canada, Harrow Research Centre, Harrow, ON, Canada N0R 1G0

*Corresponding author.

ABSTRACT

Acetylene inhibition of N₂O reduction to N₂ is traditionally used to estimate denitrification. Although NO is also produced in the denitrification process, the effect of C₂H₂ on NO production in denitrification has not previously been examined, mainly because C₂H₂ interferes with chemiluminescent analysis of NO. We used a gas-flow system to study the effect of C₂H₂ on NO and N₂O production in soil columns under denitrifying conditions and developed a method to avoid C₂H₂ interference of NO analysis. Nitric oxide, N₂O, and C₂H₂ were measured in NO^–₃-amended Brookston clay loam (fine-loamy, mixed, mesic Typic Argiaquoll) and Fox sandy loam (fine-loamy over sandy or sandy-skeletal, mixed, mesic Typic Hapludalf) with various moisture contents with and without added glucose during 50-h anaerobic incubations. In glucose-amended Brookston soil with 8% C₂H₂ added to the N₂ carrier gas, net NO production rates were increasingly inhibited after the first 24 h, and by 50 h were only 2 to 7% of the rates obtained in the absence of C₂H₂. Without glucose amendment and with C₂H₂, net NO production rates decreased by 24 to 62% in the Brookston soil. In the Fox soil inhibition occurred almost immediately on C₂H₂ addition and net NO production during 50 h was reduced by 36 to 62% with glucose amendment, and by 65 to 76% without glucose amendment. In most cases, net N₂O production rates were decreased by C₂H₂ due to the decrease in production of its precursor, NO, and in some cases, increased due to inhibition of N₂O reduction to N₂. Our data suggest that the C₂H₂ inhibition method may underestimate total denitrification rates under low water contents or high levels of C substrate.

Received for publication March 23, 1995.

This article has been cited by other articles:

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