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Characterization of the Hydrophobic Acid Fraction Isolated from a Wheat Straw Extract

Dep. of Plants, Soils, and Biometeorology, Utah State Univ., Logan, UT 84322-4820

William P. Inskeep

Dep. of Plant and Soil Science, Montana State Univ., Bozeman, MT 59717-0312

*Corresponding author (grossl{at}cc.usu.edu).

ABSTRACT

The hydrophobic acid portion (WSE-FA) was isolated from a water-soluble extract of wheat (Triticum aestivum L.) straw and chemically characterized. This material was isolated and concentrated on a column containing Amberlite XAD-8 resin. Chemical analysis of the WSE-FA included elemental composition (C, O, H, N, S, P, and ash); titrametric evaluation of carboxylic acid and phenolic functional group content; molecular weight (MW) determination by vapor pressure osmometry (VPO); gel filtration and ultrafiltration; and UV-visible, solid-state cross-polarization magic angle spinning ¹³C nuclear magnetic resonance (NMR), and Fourier-transform infrared spectroscopy. The MW of the WSE-FA was approximately 600 daltons. The ¹³C-NMR spectrum, the low MW relative to other humic substances, high E₄/E₆ ratio, and the low N content suggested that the WSE-FA was comprised of small, aromatic phenolic acids, oxidized polyphenols with exposed carboxyl groups, and saccharides that were condensed during senescence.

Contribution of the Montana State Univ. Agric. Exp. Stn., Technical Paper no. J-4036.

Received for publication December 14, 1993.