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Dep. of Agronomy and Soils, Washington State Univ., Puyallup Research and Extension Center, Puyallup, WA 98371-4998
Dep. of Agronomy, Kansas State Univ., Manhattan, KS 66506
* Corresponding author.
ABSTRACT
Flow injection analysis (FIA) is a relatively new technique with advantages over traditional automated analysis methods in precision and sample throughput. A new procedure has been developed to measure urea in soil extracts using automated FIA. This procedure utilizes the diacetyl monoxime thiosemicarbazide colorimetric method, with a modified acid reagent that increases the rate of color formation and improves the mixing of sample with reagent in narrow-diameter (0.8-mm) tubing. Sixty samples can be analyzed per hour, with a detection limit of 0.01 mg urea-N L–1. Residence time between sample injection and maximum absorbance is 130 s.
Contribution no. 90-137-J from the Kansas Agric. Exp. Stn.
Received for publication October 9, 1989.
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