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A Dilute Medium to Determine Population Size of Ammonium Oxidizers in Forest Soils

Natl. Res. Counc., US-EPA, 200 S.W. 35th St., Corvallis, OR 97333

Gray S. Henderson

School of Forestry, Fisheries and Wildlife, Agric. Bldg., Univ. of Missouri, Columbia, MO 65211

*Corresponding author.

ABSTRACT

Incubation experiments using soils from mixed oak (Quercus sp.) stands showed accumulation of nitrate but standard most probable number (MPN) assays showed no NH₄-oxidizing bacteria were present. This led to a preliminary conclusion that nitrification in these soils must be heterotrophic. However, further incubation studies indicated that NH₄-N concentrations in standard MPN medium were too high for autotrophic NH₄ oxidizers that are adapted to soils with low NH₄-N levels and exhibit low activities. Therefore, media with lower NH₄-N concentrations were tested against the standard MPN medium. Each medium was tested using both 1 mM phosphate buffer and deionized water as diluent to examine responses to small changes in buffer capacity. A 0.1 x medium used with deionized water diluent was found to best estimate populations of low-activity NH₄-oxidizing bacteria present in soils with low NH₄-N concentrations.

Contribution of the Missouri Agric. Exp. Stn. Journal no. 10760.

Received for publication April 28, 1988.

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