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Introduction of Acetylene Into Soil for Measurement of Denitrification¹

ABSTRACT

Gaseous N loss via denitrification can be measured with static flux chambers when acetylene (C₂H₂) is used to block nitrous oxide (N₂) reduction. This paper describes a method for the introduction of C₂H₂ at low concentrations into the soil by diffusion. The method utilizes an above-ground manifold connected to diffusion tubes inserted in the soil and C₂H₂ flows through the manifold at a rate of 0.33 mL s^–1. Acetylene levels in the soil atmosphere within the flux chamber site ranged from 0.16 to 2.07 mol m^–3, and the apparatus did not alter CO₂ fluxes as measured by a static chamber method.

¹ Joint contribution from the Dep. of Agronomy, Mississippi Agricultural and Forestry Station, Mississippi State, MS 39762, and Dep. of Agronomy, Cornell Univ., Ithaca, NY 14853. Journal Paper no. 6034.

² Assistant Professor of Soil Science, Mississippi State Univ., and Associate Professor of Soil Chemistry, Cornell Univ.

Received for publication February 19, 1985. Accepted for publication August 21, 1985.