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A New Method for Determination of ¹⁵N-Labeled Nitrous Oxide¹

ABSTRACT

A procedure is described that permits determination of nitrous oxide (N₂O) evolved from soils treated with ¹⁵N-labeled fertilizer. This procedure involves dilution of ¹⁵N-labeled N₂O with a known amount of unenriched dinitrogen (N₂), reduction of the N₂O to N₂ in a heated tube containing reduced copper, and determination of the ratios, ²⁹N₂/²⁸N₂ and ³⁰N₂/(²⁸N₂ + ²⁹N₂), with a dual-inlet ratio mass spectrometer. The procedure described is accurate and precise; it permits measurement of ¹⁵N-labeled N₂O without interference by CO₂; it allows calculation of the average ¹⁵N enrichment of the nitrogen pool from which this N₂O is derived; and it permits N₂O analysis of an atmospheric sample previously analyzed for ¹⁵N-labeled N₂.

¹ This study was a part of Project no. ILLU-15-0392 of the Agric. Exp. Stn., College of Agriculture, Univ. of Illinois, Urbana-Champaign. It was supported in part by the Tennessee Valley Authority.

² Research Assistant and Professor, respectively, Dep. of Agronomy, Univ. of Illinois, Urbana, IL 61801.

Received for publication March 11, 1982. Accepted for publication June 21, 1982.

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