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Published in Soil Sci Soc Am J 45:871-875 (1981)
© 1981 Soil Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Measurement of Mycorrhizal Infection in Soybeans1

G. J. Bethlenfalvay, R. S. Pacovsky and M. S. Brown2

ABSTRACT

Infection of soybean [Glycine max (L.) Merr.] roots by the vesicular-arbuscular mycorrhizal fungus Glomus fasciculatus (Thaxt. sensu Gerd.) Gerd. and Trappe was assessed throughout the ontogeny of the symbiotic association. Degree of infection was evaluated by a histological method as percent infection and colorimetrically as mg chitin/g mycorrhiza. Correlation of data by the two methods was highly significant (r = 0.99) below 60% infection and not significant (r = 0.62) at higher levels. Assessment of infection by the histological method did not yield significantly different data above 60% infection. Data by the colorimetric method were statistically distinct at all levels of infection. The effects of biological and chemical contaminants on the colorimetric assay were determined. The fungal component of mycorrhizae was compared to purified chitin standards. Use of chitin for standard curves in the absence of degradation products from uninfected root materials significantly (p < 0.05) overstated the degree of infection. Colorimetric determination of fungal infection in mycorrhizae is recommended when high levels of infection are expected.

NOTES

1 Contribution from the Western Regional Research Center, USDA-ARS, Berkeley, CA 94710.

2 Research Plant Physiologist, Research Assistant, and Research Chemist, respectively, USDA-ARS, Berkeley, CA 94710.

Received for publication December 23, 1980. Accepted for publication April 28, 1981.






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