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Extraction of Phosphatase, Urease, Proteases, Organic Carbon, and Nitrogen from Soil¹

ABSTRACT

Extractions of three soils at different incubation times demonstrate that 0.14M sodium pyrophosphate at pH 7.1 extracts phosphatase, urease, casein, and benzoylarginamide hydrolyzing proteases. Extraction yields of phosphatase and casein-hydrolyzing proteases are remarkably high. The most efficient length of extraction depends both on the type of soil and on the selected enzyme.

Correlation analyses of extracted enzymes and extracted C and organic N content generally show a significant correlation (p = 0.05 and 0.01).

¹ Contribution from the Laboratory for Soil Chemistry, C.N.R., via Corridoni, 78, Pisa, Italy. Scientific Paper no. 125. Partly based on the junior author's M.S. thesis.

² Soil Scientists and Graduate Student, respectively.

Received for publication August 13, 1979. Accepted for publication June 3, 1980.

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