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An Automated Method for the Determination of Orthophosphate in the Presence of Labile Polyphosphates¹

ABSTRACT

An automated method for determining orthophosphate in the presence of labile polyphosphates is described for the range 0–10 ppm orthophosphate-P. Interference by pyrophosphate and tripolyphosphate at concentrations of 10 and 200 ppm is < 1% of their P content. The precision of the method was satisfactory (the calibration curve has a correlation coefficient > 0.999 and a standard deviation around the regression line of ± 0.06), baseline drift was small, and only small changes in absorbance for the same standards over the course of 6 hours were observed. Analytical results obtained via the automated method for soil extracts and prepared mixtures compare favorably with analyses performed via the original manual method. One of the adaptations described also improves the original manual method.

¹ Contribution from the Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel. no. 160-E, 1979 series.

² Scientist, Div. of Soil Chemistry and Plant Nutrition, Institute of Soils and Water, Agric. Res. Org., The Volcani Center, Bet Dagan, Israel.

Received for publication May 21, 1979. Accepted for publication September 18, 1979.