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A Reevaluation of Phosphorus-32 and Resin Methods in a Calcareous Soil¹

ABSTRACT

Four experiments were run to evaluate the resin and ³²P methods as valid laboratory methods in a calcareous soil. Recovery of four levels of added KH₂PO₄, after several cycles of wetting, drying, and mixing was evaluated. Total-exchangeable P (TEP₂₄) was determined by the carrier-free method, and resinextractable P by equilibrating 1 g soil sample with 2 g resin overnight. Equilibrium of ³¹P and ³²P on the soil-solid phase was evaluated by measuring specific activities with time. Different soil-solution ratios, using water and 0.01M CaCl² as extractants, were evaluated to determine their effects on the total exchangeable P. Water and four different salts at three different molarities were used as extractants with 1 and 2 g resin to determine total released P in solution and on the resin.

The recovery experiment showed a high correlation between added and recovered P, using both TEP₂₄ and resin methods; the time-equilibration study indicated a lack of complete equilibrium between ³¹P and ³²P after 2 hours shaking, and a near complete or complete equilibrium at 24 hours; different soil-solution ratios did not affect total exchangeable P, and water extracted the most resin-P and 0.01M CaSO₄ the least (roughly one-half of pure water):

¹ Contribution from the USDA, Science and Education Administration in cooperation with the Colorado State Univ. Experiment Station. Scientific Series No. 2253.

² Soil Scientists, USDA-SEA, Agron. Dept., Colorado State Univ., Fort Collins, CO 80523.

Received for publication October 25, 1977. Accepted for publication August 31, 1978.

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