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Microbial Epimerization of Myo-inositol to Chiro-inositol in Soil¹

ABSTRACT

¹⁴Carbon-(1,2,3,5/4,6)-inositol (¹⁴C-myo-inositol) was incorporated into an Andosol sandy loam soil and incubated for a 12-day period at 70% field moisture capacity under sterile and nonsterile soil conditions. Comparison by thin-layer chromatography of an isolated metabolite with an authentic standard and infrared spectrometry were used to identify the metabolite as ¹⁴C-(1,2,4/3,5,6)-inositol (¹⁴C-chiroinositol) of unknown optical activity. The ¹⁴C-chiro-inositol was identified in the biologically active soil. No epimerization of ¹⁴C-chiroinositol was observed in sterile soil. The abundance of the ¹⁴C-chiroinositol was approximately 4% relative to that of the parent compound, as determined by liquid scintillation counting of the two chromatographically separated compounds.

¹ Contribution from the Dep. de Agricultura, Instit. Nacional de Energia Nuclear, Insurgentes Sur No. 1079, Apartado Postal No. 27-190, México 18, D.F., México.

² Research Soil Biochemist and National Institute of Nuclear Energy Research Fellows, respectively.

Received for publication December 27, 1976. Accepted for publication March 28, 1977.