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Further Evidence for the Inability of the Kjeldahl Total Nitrogen Method to Fully Measure Indigenous Fixed Ammonium Nitrogen in Subsoils¹

ABSTRACT

Three methods of Kjeldahl total N determinations in soils were compared. Method 1 involved a pretreatment of the soil with a HF-acid mixture and resulted in higher N values than methods 2 or 3 which involved a long period of digestion and a modification of a Gunning method, respectively. Differences in total N values obtained by the three methods were greatest for subsoils. The inability of methods 2 and 3 to measure all of the indigenous fixed NH₄⁺-N resulted in low N values and erroneously high C:N ratios in the subsoil.

¹ Published with the approval of the Director as Paper no. 3277 of the Journal Series, Nebr. Agr. Exp. Sta., Lincoln 68503. Research reported was conducted under Project no. 12–73.

² Former NSF Trainee, now Graduate Assistant at University of Illinois and Associate Professor at University of Nebraska, respectively.

Received for publication November 11, 1971. Accepted for publication January 12, 1972.