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Arylsulfatase Activity of Soils¹

ABSTRACT

The detection of arylsulfatases (enzymes that hydrolyze aryl sulfates) in soils is reported, and a simple method of assaying soil arylsulfatase activity is described. This method involves colorimetric estimation of the p-nitrophenol released by arylsulfatase activity when soil is incubated with buffered (pH 5.8) potassium p-nitrophenyl sulfate solution and toluene at 37C for 1 hour, and it is rapid, sensitive, and precise. The procedure used to extract p-nitrophenol stops arylsulfatase activity, develops the yellow color used to estimate p-nitrophenol, and gives quantitative recovery of this compound. Studies of the properties of the arylsulfatases in soils are reported.

¹ Journal Paper no. J-6347 of the Iowa Agr. & Home Econ. Exp. Sta., Ames. Project no. 1070. This work was supported in part by The Sulphur Institute. Presented before Div. S-3, Soil Science Society of America, Washington, D.C., Nov. 9, 1967.

² Research Associate and Professor, respectively, Department of Agronomy, Iowa State University, Ames.

Received for publication August 19, 1969. Accepted for publication October 2, 1969.