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Organic Phosphorus and Inositol Phosphates in Molecular Size Fractions of a Soil Organic Matter Extract¹

ABSTRACT

Soil organic matter extracted with the chelating resin Dowex A-1, was fractionated into three fractions on a column of Sephadex G-75. The first fraction, which has an approximate lower molecular weight limit of 50,000, contained 36% of the total organic P in the extract. The second fraction, with intermediate molecular weights in the range 1,000–50,000, contained 31% of the P. The third fraction, which was considered below the fractionation range and therefore is below 1,000 molecular weight, contained 7.8% of the organic P in the extract. Inositol phosphates were detected in the second and third fractions but not in the first fraction. Inositol phosphates in the third fraction were considered to be in the free form, while those in the second fraction must be in either a polymer form or bound to other organic compounds.

¹ Contribution from the Dep. of Soil Science, University of Guelph, Guelph, Ontario, Financial assistance granted by the National Research Council of Canada and the Ontario Dep. of Agr. and Food is gratefully acknowledged. Presented before Div. S-3, Soil Science Society of America, at New Orleans, La., Nov. 12, 1968.

² Research Assistant and Associate Professor respectively.

Received for publication July 29, 1969. Accepted for publication October 6, 1969.