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Ion Behavior in Yeast. II. Kinetics¹

ABSTRACT

According to popular hypotheses, ion entry into living cells is a carrier-mediated process which is under the control of the cell. Kinetic analyses of ion entry, based upon the Michaelis-Menten treatment, have been widely accepted as the most convincing evidence for carrier-based hypotheses. In this investigation we reconsider the kinetics of ion entry into yeast cell (Saccharomyces cerevisiae Meyer ex Hansen) and show that the data, in fact, cannot be reasonably reconciled with a carrier model. The data are conversely shown to be in good agreement with an equilibration process in which the cell behaves like an ion exchanger enclosed within a rate-limiting barrier and through which ions move simply by diffusion.

¹ Technical contribution no. 922, Chemistry Dept., Montana State University, Bozeman, Mont. The investigation was partially supported by a grant from the National Science Foundation.

² Professor.

Received for publication July 6, 1968. Accepted for publication January 14, 1969.