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A Simple Technique for Preparing and Maintaining Sterile Soils for Plant Studies¹

ABSTRACT

The proposed method depends upon the antimycotic activity of propylene oxide and the permeability of polyethylene. Inside a warmed pressure cooker, the epoxide diffuses rapidly through a plastic bag, sterilizing the moist soil contained. Release of pressure reverses diffusion. Detoxication is completed under vacuum at 45C. The bag then serves as a chamber for the storage of sterile soil or for the sterile culture of plants.

¹ Vermont Agr. Exp. Sta. Journal Article no. 180. Presented before Div. S-3, Soil Sci. Soc. Amer., Stillwater, Okla., Aug. 21, 1966. The authors with to thank Dr. K. D. Fisher, Botany Dept., Univ. of Vermont, for his advice and help in this study.

² Associate Professor of Agronomy, Univ. of Vermont, Burlington, and Graduate Research Fellow, respectively. Junior author is now a Research Instructor at Virginia Polytechnic Inst., Blacksburg.

Received for publication November 14, 1966. Accepted for publication January 12, 1967.