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Nitrosation of Soil Organic Matter: II. Gas Chromatographic Separation of Gaseous Products¹

ABSTRACT

Gas chromatographic techniques were developed for separating and determining inorganic gases in experimental systems dealing with losses of soil N. A single column, consisting of acid-washed chromatographic-grade coconut charcoal, gave excellent resolution of H₂, N₂, CO, CO₂, N₂O, and NO (or O₂). The sensitivity of the method for NO and CO was improved considerably by blocking sorption sites responsible for irreversible retention of these gases. Alkyl nitrites did not interfere with the separations.

¹ Contribution from the Department of Agronomy, University of Illinois, Urbana. Supported by regional research funds as a contributing project to Regional Project NC-55 and by the Illinois Research Board. Presented before Div. S-3, Soil Science Society of America, Kansas City, Mo., Nov. 16, 1964.

² Professor of Soil Chemistry and Research Associate, respectively. The junior author is now Assistant Soil Scientist, Georgia Experiment Station, Experiment, Georgia.

Received for publication March 21, 1966. Accepted for publication May 25, 1966.