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Published in Soil Sci Soc Am J 30:217-220 (1966)
© 1966 Soil Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Decomposition Products of Carbon-14 Labelled Rye Tissue in a Peat Profile1

K. S. Chahal, J. L. Mortensen and F. L. Himes2

ABSTRACT

After rye (SECALE CEERALE) tissue, labelled with C14, had been incubated with a peat soil, the soil was extracted with various solvents. One fractionation procedure was the proximate analysis method according to Waksman and Stevens (15). The other procedure was a comparison of the components extracted by 0.1M Na4P2O7, 0.5N NaOH, and 6N HCl.

In the proximate analysis, the distribution of total carbon in benzene-methano (10:1), 2% HCl, 80% H2SO4, and insoluble portion was 1.9, 30.8, 17.2, and 50.1%, respectively. The respective distribution of C-14 was 40.3, 20.5, 13.4, and 25.8%. The fraction containing the smallest proportion of carbon had the largest proportion of C-14.

The 6N HCl extract was separated into more discrete fractions by gel infiltration than the extracts of 0.1M Na4P2O7 and 0.5N NaOH. However, the NaOH removed a greater proportion of the radioactive materials.

NOTES

1 Contribution from Department of Agronomy, The Ohio State University. Taken in part from the Ph.D. Diss., of the Senior Author. The Ohio State Univ., 1963. Presented before Div. S-3, Soil Science Society of America, Kansas City, Nov. 1964.

2 Formerly Assistant, now Assistant Professor of Agronomy Tuskegee Institute, Alabama, and Professor, and Assistant Provessor, The Ohio State University, respectively.

Received for publication June 28, 1965. Accepted for publication November 23, 1965.






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