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Published in Soil Sci Soc Am J 27:312-316 (1963)
© 1963 Soil Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Chemical Distribution of Added Nitrogen in Soils1

H. H. Cheng and L. T. Kurtz2

ABSTRACT

The distribution of added nitrogen (N) among the various components of soil N was determined, using isotopic tracing techniques. Three lysimeter soils, previously fertilized with N15-enriched N, were studied. Methods of fractionating soil N were modified to permit estimation of the isotopic ratio as well as the N content of the different fractions. Soil N was fractionated into: exchangeable NH4+ plus NO3-, fixed NH4+, N in acid hydrolyzate, and acid-insoluble humin N. The N in acid hydrolyzate was further separated into the alkali-labile N and the alkali-stable N. The alkali-labile N consisted of hydrolyzed NH4+ and amino sugar N. The alkali-stable N was divided into soluble N, which included the amino acid N, and acid-soluble but alkali-insoluble humin N. Validity of the classification was discussed. Usefulness of N15 data was also evaluated. Estimation of the distribution of added N showed that over 90% of added N in soils was found in the hydrolytic products of soil organic matter, mainly as amino acid N, amino sugar N, and other soluble N.

NOTES

1 Contribution from the Department of Agronomy, University of Illinois, Urbana. Presented before Div. II, Soil Science Society of America, Nov. 27, 1961, at St. Louis, Mo. Part of senior author's Ph.D. thesis, University of Illinois, Urbana, 1961.

2 Research Associate and Professor of Soil Fertility, respectively, Department of Agronomy, University of Illinois. Present address of senior author is Department of Agronomy, Iowa State University, Ames.

Received for publication June 4, 1962. Accepted for publication October 16, 1962.






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