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Soil Nitrogen Complexes: II. Electrophoretic Separation of Organic Components¹

ABSTRACT

Extracts of soil organic matter solubilized by 0.1M Na₄P₂O₇ and hydrolyzed by 0.5N NaOH, 6N HCl, and 0.1M Na₄P₂O₇ + 0.5N NaOH were electrically desalted and subjected to continuous flow paper electrophoresis. Determination of organic matter, total nitrogen, -amino nitrogen and amino sugar in the collection tubes revealed the presence of nitrogen-containing components having different electrophoretic mobilities.

NaOH and HCl hydrolysates contained several nitrogenous components in which -amino nitrogen and amino sugars were detected. These hydrolysates also contained a brown-colored component which contained most of the organic matter. The Na₄P₂O₇ extract, which exhibited considerable loss of organic nitrogen during desalting, contained one major component in which most of the nitrogen and brown material was contained.

A fluorescent component was associated with the brown-colored component in each extract.

¹ Journal paper No. 19–60 of the Ohio Agr. Exp. Sta., published with permission of the Director as a collaborator under North Central Region Cooperative Project (NC-17) entitled "Value of Organic Matter and Soil and Crop Management Practices in Improving Soil Structure and Productivity." Taken in part from the M.S. thesis of the Senior Author, Ohio State University, 1959. Presented before Div. III, Nov. 16, 1959, at Cincinnati, Ohio.

² Research Assistant and Associate Professor, respectively, Ohio Agr. Exp. Sta., and the Ohio State University.

Received for publication March 21, 1960. Accepted for publication April 18, 1960.