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Elimination of Interference by Phosphorus and Other Elements in the Flame Photometric Determination of Calcium and Magnesium in Plant Tissue¹

ABSTRACT

A rapid, accurate flame photometric method was devised for determination of Ca and Mg in plant tissue. Interferences by P and any Fe and Al that might be present were eliminated by addition of FeCl₃ to the acid plant ash extract and precipitation of P, Fe and Al by neutralizing to pH 5.6 with NH₄OH. A positive interference by K was corrected by addition of 500 ppm. K to all solutions. NH₄OAc was added to prevent clogging of the burner capillary by the NH₄Cl solution. Results obtained with the new method agreed well with those obtained with AOAC methods.

Contribution from the Soils Department, University of Wisconsin, Madison. Published with the permission of the Director of the Wisconsin Agr. Exp. Sta. This work was supported in part by a grant from the American Potash Institute, Inc.

² Soil Scientist, Union Research Center, Southeastern Forest Experiment Station, Union, S. C., formerly Research Assistant, University of Wisconsin; and Associate Professor, respectively.

Received for publication November 2, 1959. Accepted for publication November 12, 1959.