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ABSTRACT
A procedure was developed for the quantitative determination of inositol hexaphosphates in extracts of soils and manures. The inositol hexaphosphates were separated from other phosphorus compounds by anion-exchange chromatography in a simplified adaptation of the technique developed originally by Smith and Clark. Dilute hydrochloric acid extracts of manures can be added directly to the exchange columns, but extracts of soils obtained by the usual acid-alkali extraction procedure must be given a preliminary treatment to remove organic matter that otherwise would flocculate under the acid conditions in the exchange columns.
1 Contribution from the Department of Agronomy, Iowa State College. Journal Paper No. J-3259 of the Iowa Agr. and Home Econ. Exp. Sta., Ames, Iowa. Project No. 1183. Presented before Div. II, Soil Science Society of America, Nov. 15, 1956, at Cincinnati, Ohio.
2 Former Graduate Assistant (now Associate Professor of Soils, Texas A. & M. College), and Professor of Soils, respectively. The senior author is indebted to the Agricultural Institute of Canada and the Research Council of Ontario for financial support during a part of the time this work was underway.
Received for publication January 7, 1958. Accepted for publication March 21, 1958.
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