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The Determination of Polyuronides in Soils with Carbazole¹

ABSTRACT

A method is described for the determination of uronic acids in soil organic matter using a colorimetric method in which a complex is formed with carbazole. Ferric iron interferes in this procedure in amounts over 20 ppm. This interference may be removed by reducing the iron with stannous chloride. This method is rapid and reproducible and can be readily used to follow the rapid breakdown and production of polyuronides in soils. Data showing results obtained with this method are included.

¹ Published as Miscellaneous Paper No. 250 with the approval of the Director of the Delaware Agr. Exp. Sta. Contribution of the Department of Agronomy. Funds for this research were provided by Andelot, Inc., through the University of Delaware Research Foundation.

² Assistant Research Professor, Graduate Assistant, and Associate Professor of Agronomy, respectively, University of Delaware.

Received for publication August 31, 1956. Accepted for publication October 9, 1956.