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Isolation and Identification of Some Amino Compounds in Soils¹

ABSTRACT

An isolation experiment was conducted to obtain sufficient amounts of all of the amino acids in a soil hydrolysate to permit their identification. This was done by a selective separation on a large column of Dowex 50, in which elution was carried out by hydrochloric acid. The identification of the various compounds was accomplished by the application of specific tests, by paper partition chromatography, and by ion exchange chromatography on a sodium column of Dowex 50, using the procedure of Moore and Stein.

Twenty-nine ninhydrin-reacting compounds were identified positively as products of acid hydrolysis of soils, one compound was identified tentatively, and three others were isolated but not characterized. In addition to the compounds previously verified, the following were confirmed: cysteic acid, N-acetyl-D-glucosamine, methionine sulfone, , -diaminopimelic acid, -amino-n-butyric acid, methionine sulfoxide, methionine, cystine, and ornithine. A discussion is given concerning the natural occurrence of these compounds in soils.

¹ Contribution from the Department of Agronomy, Illinois Agr. Exp. Sta., Urbana, Ill. Published with the approval of the Director.

² Assistant Professor in Soil Biology. Appreciation is expressed to Miss Mary Louise McKenzie, Mr. Uldis Blukis, and Mr. John B. Duncan for performing many of the determinations.

Received for publication May 11, 1955.