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ABSTRACT
This study was aimed to ascertain (a) the natural occurrence of R. phaseoli in certain soils, (b) whether any relationships existed between types of nodulation and efficiencies of the strains, and (c) homogeneity of reactions among strains isolated from the same nodule.
Variations among the strains in cultural and fermentative characteristics were marked, yet, on the whole, the strains isolated from the same nodules showed more uniformity in litmus milk reactions and ability to utilize the same carbohydrates than did those obtained from different nodules.
From a practical point of view bean rhizobia were not abundant in any of the soils tested. Rhizobia isolated from different nodules on the same plant varied widely in efficiency. Of the 85 strains isolated from nodules formed on plants grown in soil-vermiculite mixtures, only 12 were highly effective. Differences in plant growth response to strains isolated from suspensions of the same nodule were insignificant.
1 Contribution from the Department of Bacteriology, University of Wisconsin, Madison 6. Published with the approval of the Director, Wisconsin Agricultural Experiment Station. Presented before Section III, Soil Science Society of America, State College, Pa., August 29, 1951.
2 Graduate student, Professor of Bacteriology, and Professor of Soils, respectively.
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