Fig. 1. Digital image processing of fluorescent in situ hybridization (FISH)–microautoradiography (MAR). Separate images for each color channel and the phase contrast counter image can be composited into a single image to simplify detection of cells that consume radioisotopic substrates. Stain DAPI (excitation = 385–400 nm, emission = 450–465 nm); probe EUB338-I, II, III (stain CY3; excitation = 530–560 nm, emission = 573–648 nm).